Journal: bioRxiv

Article Title: Single cell analysis reveals molecular traits of pediatric lymphoma resistant subclones

doi: 10.64898/2026.04.21.719850

Figure Lengend Snippet: Progenitor-like lymphoma cells are potentially chemoresistance: A. Integration of all cancerous pBL single cells. BCR status shown in color. B. Expression level of genes involved/targeted by R-CHOP regiment (killing) or related to chemoresistance (resist) in cancerous pBL single cells samples. C. Integration of all cancerous pTLBL single cells. TCR status shown in color. D. Expression level of genes involved/targeted by CHOP regiment (killing) or related to chemoresistance (resist) in cancerous pTLBL samples. E. (Left) expression correlation between TUBB and other genes in pBL. Top positive (in blue) and negative (in red) correlated genes were highlighted together with MSI2 . (Right) top enriched terms (gene ontology biological process, GOBP; WikiPathway, WikiPath) for top 300 genes negatively correlated with TUBB expression. F. Highlighted gene expression negatively correlated with TUBB . G. Same as E. but for pTLBL. H. Same as in F. but referred to pTLBL cancerous cells. I. Apoptosis assay for Raji, SUP-T1 and Jurkat cell lines upon MSI2 inhibitor treatment. Cells were incubated for 48h at designated concentrations. J. Schematics of NSG xenograft model utilizing Raji cell line. MSI2 inhibitor was administered intratumorally every 2 days starting from day 10. Experiment end point is represented by tumour reaching 1cm3. K. Tumor volume evaluated at each time point. Statistical analysis performed utilizing 2 way Anova - Šídák’s multiple comparisons test (untill D24). L. Percentage of survival with and without MSI2 inhibitor treatment referred to J. , curve comparison analyzed with Logrank (Mantel-Cox) test.

Article Snippet: Raji, Jurkat and SUP-T1 cell lines were cultured with complete RPMI1640 with 5, 10, 20 μM of MSI2 inhibitor (Ro 08-2750, # HY-108466, MedChemExpress) or DMSO (vehicle) for 48h.

Techniques: Expressing, Gene Expression, Apoptosis Assay, Incubation, Comparison

Journal: Cell Reports

Article Title: Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection

doi: 10.1016/j.celrep.2026.117090

Figure Lengend Snippet: In virion RNA interactome capture (ivRIC) reveals the composition of the genomic ribonucleoprotein packaged within HIV-1 virions (ivRNP) (A) Schematic representation of the ivRIC protocol. Samples and controls are depicted in . (B) Western blotting of the viral particles before and after sucrose cushion purification using antibodies against markers of extracellular vesicles (CD45, CD43, and SERINC3) and viral proteins; representative gel of n = 3. (C) Western blotting of the whole-cell lysates (WCLs), inputs, and eluates of a representative ivRIC experiment performed in CD4 + T-lymphocytic (SupT1) cells. Asterisk marks unspecific bands; representative gel of n = 3. (D) Schematic representation of the proteolysis of the Gag polyprotein. MA , matrix; CA , capsid; NC , nucleocapsid. (E) Relative proportion of the HIV-1 gRNA in ivRIC eluates estimated by absolute RT-qPCR; n = 3; the line within the box represents the mean and error bars ± standard deviation (SD). (F) Analysis of oligo(dT) isolated RNA from sucrose purified HIV-1 mCherry-Nef particles by RNA-sequencing; n = 3. (G and H) Volcano plots of the quantitative proteomic analysis of the ivRIC eluates from virions produced by SupT1 cells; n = 4. Red and dark blue dots are proteins enriched with 1% FDR, while orange and cyan dots are proteins enriched with 10% FDR. Gray dots are non-enriched proteins. E , eluate. (I) Venn diagram showing the overlapping between the UV irradiated vs. non-irradiated samples and HIV-1-infected versus mock-infected comparisons. (J) Bar plots showing the proportion of ivRBPs in the ivRNP annotated with RNA- and DNA-binding (GO terms); virus-related (GO terms), and HIV-1-related (text-mining) functions. Related to and .

Article Snippet: The following human cell lines are available in cell culture collections or commercially: T-lymphoblast SupT1 (male; ECAAC, #95013123), T-lymphocyte Jurkat Flp-In (male; Thermo Fisher Scientific, # R76207 ), embryonic kidney HEK293 Flp-In T-REx (female; Thermo Fisher Scientific, # R78007 ), HeLa (female; ATCC, #CCL-2), Huh-7 (male; a gift from Matthias W Hentze) and HEK293T (female; ATCC, #CRL-3216).

Techniques: Western Blot, Purification, Quantitative RT-PCR, Standard Deviation, Isolation, RNA Sequencing, Produced, Irradiation, Infection, Binding Assay, Virus

Journal: Cell Reports

Article Title: Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection

doi: 10.1016/j.celrep.2026.117090

Figure Lengend Snippet: The Rev protein-protein interactome and its similarities with the ivRNP (A) Schematic representation of the HIV-1 R-E-Rev-Flag-Myc replicon. (B–D) Western blotting showing viral protein expression in CD4 + T lymphocytic cells (SupT1) infected with HIV-1 R-E-Rev-Flag-Myc (B), HIV-1 R-E-Rev-Halo (C), or HIV-1 R-E-ΔRev (D) relative to the parental HIV-1 R-E-mCherry-Nef . hpi , hours post-infection; representative gels of n = 3. (E) Schematic representation of the Rev protein-protein interaction experiment, using chimeric pseudotyped viruses to infect CD4 + T lymphocytic cells (SupT1). (F) Western blot of inputs and eluates of Rev IP using Flag and p24 antibodies. (G)Volcano plot of the Rev-Flag-Myc immunoprecipitation (IP) versus the untagged control (Rev WT); n = 3. Red and dark blue dots are proteins enriched with 1% FDR, while orange and cyan dots are proteins enriched with 10% FDR. Gray dots are non-enriched proteins. (H) Bar plots showing the proportion of Rev interactors annotated with RNA- and DNA-binding (GO terms); virus-related (GO terms), and HIV-1-related (text-mining) functions. (I) Proportion of novel and previously reported Rev interactors in the Rev interactome. (J) Pie chart showing the proportion of proteins classified as novel Rev interactors and previous annotations in HIV-1 NCBI database. (K) Venn diagram showing the overlapping between the ivRIC (ivRNP) and the Rev IP (Rev interactome) experiments. (L) Scatterplots comparing fold changes in the Rev interactome and the ivRIC experiment. Related to .

Article Snippet: The following human cell lines are available in cell culture collections or commercially: T-lymphoblast SupT1 (male; ECAAC, #95013123), T-lymphocyte Jurkat Flp-In (male; Thermo Fisher Scientific, # R76207 ), embryonic kidney HEK293 Flp-In T-REx (female; Thermo Fisher Scientific, # R78007 ), HeLa (female; ATCC, #CCL-2), Huh-7 (male; a gift from Matthias W Hentze) and HEK293T (female; ATCC, #CRL-3216).

Techniques: Western Blot, Expressing, Infection, Immunoprecipitation, Control, Binding Assay, Virus

Journal: Cell Reports

Article Title: Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection

doi: 10.1016/j.celrep.2026.117090

Figure Lengend Snippet: Effect of ivRBPs in viral gene expression and virion production and infectivity (A) Representation of the experimental design. (B) Flow cytometry analysis of WT and KO SupT1 cells infected with HIV-1 mCherry-Nef for 48 h. y axis show the percentage of KO cells expressing mCherry as compared to WT cells (dashed line). (C) Western blotting analysis of WT, PURA, and PURB KO SupT1 cells infected with HIV-1 mCherry-Nef for 24 h; a representative gel of n = 3. (D) RT-qPCR analysis of the HIV-1 gRNA in the supernatant of WT- and KO-infected SupT1 cells from (B). (E) Viral particles purified in (D) from KO and WT cells were used to infect WT SupT1 cells upon normalization by gRNA levels. mCherry positive cells were quantified by flow cytometry at 48 hpi. For (B), (D), and (E), n = 4; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. (B), (D), and (E) show box-and-whisker plots, in which the boxes represent the interquartile range (Q1–Q3) with the median indicated, and the whiskers denote the minimum and maximum values. (F) Flow cytometry analysis of HEK293 WT cells infected at MOI 1 with single round HIV-1 R-E-mCherry-Nef produced from HEK293 Flp-In T-Rex WT or PURA KO cells; n = 3. (G) Analysis of early, intermediate, and late reverse transcription (RT) products in HEK293 Flp-In T-Rex WT cells infected with normalized single round HIV-1 R-EmCherry-Nef particles produced in HEK293 Flp-In T-Rex WT or PURA KO cells; n ≥ 3. (H) Analysis of early, intermediate, and late HIV-1 RT products in HEK293 Flp-In T-Rex WT or PURA KO cells infected with normalized single round HIV-1 R-E-mCherry-Nef particles produced in WT cells; n = 4. (I) Flow cytometry analysis of HEK293 Flp-In T-Rex WT cells infected with normalized single round HIV-1 R-E-mCherry-Nef particles produced in Huh-7 WT or PURA KO cells; n = 3. (J) Analysis of early, intermediate, and late RT products at 8 hpi in HEK293 Flp-In T-Rex WT cells infected with HIV-1 R-E-mCherry-Nef produced in Huh-7 WT or PURA KO cells; n ≥ 4. (F–J) Data is mean ± standard error of the mean (SEM), including individual data points. For all panels, ∗ p < 0.05, ∗∗ p < 0.01. Related to and .

Article Snippet: The following human cell lines are available in cell culture collections or commercially: T-lymphoblast SupT1 (male; ECAAC, #95013123), T-lymphocyte Jurkat Flp-In (male; Thermo Fisher Scientific, # R76207 ), embryonic kidney HEK293 Flp-In T-REx (female; Thermo Fisher Scientific, # R78007 ), HeLa (female; ATCC, #CCL-2), Huh-7 (male; a gift from Matthias W Hentze) and HEK293T (female; ATCC, #CRL-3216).

Techniques: Gene Expression, Infection, Flow Cytometry, Expressing, Western Blot, Quantitative RT-PCR, Purification, Whisker Assay, Produced, Reverse Transcription

Journal: Frontiers in Microbiology

Article Title: Postbiotics from lactic acid bacteria as promising alternatives against urinary tract pathogens

doi: 10.3389/fmicb.2026.1741001

Figure Lengend Snippet: Caenorhabditis elegans biosafety. C. elegans N2 viability was determined after 24 h exposure to CFS from L. paraplantarum CRL 1905 (A) . C. elegans N2 locomotor activity (%) was measured after 24 h of CFS from L. paraplantarum CRL 1905 exposure using the Worm Motility Tracker (WMT). Data represent the average locomotor activity of nematode populations from three replicate wells over time (B) . C. elegans N2 reproduction (%) in the presence of CFS from L. paraplantarum CRL 1905 was evaluated at 24 h by counting eggs and larvae (C) . Controls included worms treated with MRS and S medium, and was considered as 100% locomotor activity or expressed as 100% reproduction. Data are the result of three independent experiments and are expressed as mean ± SD with p ≤ 0.05. ***p ≤ 0.001, ****p ≤ 0.0001 ( n : 180–240 worms per treatment).

Article Snippet: Five lactobacilli species, isolated from different sources, were tested for their potential antimicrobial activity: L. paraplantarum CRL 1905, L. brevis CRL 1942, L. fermentum CRL 973, L. helveticus ATCC 15009 and L. acidophilus ATCC 4356.

Techniques: Activity Assay

Journal: Frontiers in Microbiology

Article Title: Postbiotics from lactic acid bacteria as promising alternatives against urinary tract pathogens

doi: 10.3389/fmicb.2026.1741001

Figure Lengend Snippet: Effect of CFS from L. paraplantarum CRL 1905 on C. elegans survival during S. aureus Sa1 (A–C) and P. aeruginosa Pa2 (D–F) infection. Worms were treated with different concentrations of CFS before (A,D) , during (B,E) , or after infection (C,F) . without CFS, CFS 12.5%, CFS 25%, and CFS 50%. Survival was monitored over time, and curves were generated using the Kaplan–Meier method. Data are the result of three independent experiments. Statistical comparison was performed using the log-rank test. Differences were considered significant when p ≤ 0.05. **** p ≤ 0.0001.

Article Snippet: Five lactobacilli species, isolated from different sources, were tested for their potential antimicrobial activity: L. paraplantarum CRL 1905, L. brevis CRL 1942, L. fermentum CRL 973, L. helveticus ATCC 15009 and L. acidophilus ATCC 4356.

Techniques: Infection, Generated, Comparison